major difference to what? minutes or performance.... or both?!
Try using "strictly follow depth chart" and not put any plaerys as subs or backups. Your starters should all play 48 minutes.
I can't quite recall when it happened, but I've had players subbed out in a scrimmage like this, I think I was playing look inside (so slightly increased pace).On the other hand, my C with strong stamina only gave 1 minute to the backup with pitiful one, again with strictly follow depth chart, but playing look inside (so slightly decreased pace).